WIKA

Bioprocessing

Bioprocesses are used to manufacture products in which one or more biotechnological techniques are applied. Living organisms, or their products, are used to modify living or non-living matter for the production of goods. Application areas include the production of bacterial and viral vaccines, toxoids and similar products, such as allergen extracts, sera, plasmas and other blood derivatives, for human or veterinary use.

GMP ("good manufacturing practice") compliant production and the avoidance of any risks for the consumer of the end product have the highest priority. Measurement technology from WIKA delivers process-specific information which enables high-quality production. For this, the safety, the hygienic requirements in the design of the measuring instruments and the connection of the sensors to the bioprocess equiptment are fulfilled optimally. Via the global sales and service network, experienced employees are always available to our customers for the selection and commissioning of our instrumentation solutions and also for calibration and maintenance operations.

Biotechnology × 2

Bioreactors

Bioreactors
3.1

Separator

Separator
10

Calibration and service centre

Calibration and service centre
3.2

Filtration

Filtration
1

Tanks

Tanks
3.3

Chromatograph

Chromatograph
5

Formulation

Formulation
4

Mobile tanks

Mobile tanks
6

Fill & finish

Fill & finish
7

CIP

CIP
8

SIP

SIP
9

WFI preparation

WFI preparation
Biotechnology
Minimise selection

Tanks

The pharmaceutical vessels can be used for a variety of different purposes. Among other things, they serve as preparation vessels, buffer tanks, mixing tanks, intermediate tanks or for harvesting or inactivation. Depending on the application, the tanks are fitted with insulation as well as heating and cooling jackets and, if necessary, a stirrer. The diverse tasks of the tanks require a measurement technology to match. WIKA offers a wide range of hygienic instrumentation for level measurement, overpressure and temperature monitoring in the tanks.


Tanks × P

Pressure blanket

Pressure blanket
L

Tank level

Tank level
T

Temperature sensor

Temperature sensor
P

Pressure indication

Pressure indication
T

Temperature display

Temperature display
P

Hydrostatic pressure

Hydrostatic pressure
W

Load cells

Load cells
Tanks
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The following 32 products correspond to your selection. You haven't found the product? Please contact us.

Bioreactors

The upstream process starts with thawing a vial from the cell bank and adding it to a small amount of medium, prepared in the media and buffer-preparation area of the production facility. The cells are then cultivated in bioreactors, under conditions that are optimal for them. Gradually, the cells are transferred to larger bioreactors with growth medium. This process is repeated ("scale-up") until the final volume of the bioreactor is reached. Some of the largest final volumes are 20,000 litres and more. Due to the huge costs associated with scale-up, it is important to understand the impact of process variables such as temperature and pressure.

WIKA offers specially developed instrumentation for the various process parameters around the bioreactors in the upstream process. These are, for example, level measurement with foaming media or overpressure and temperature monitoring, both in the bioreactor and for the heating jacket.


Bioreactors × T

Supply line temperature

Supply line temperature
P

CIP pressure

CIP pressure
L

Liquid phase level

Liquid phase level
P

Supply line pressure

Supply line pressure
P

Pressure indication

Pressure indication
T

CIP temperature

CIP temperature
T

Bioreactor temperature

Bioreactor temperature
P

Pressure blanket

Pressure blanket
P

Heating jacket pressure

Heating jacket pressure
T

Bioreactor temperature

Bioreactor temperature
P

Outlet line pressure

Outlet line pressure
P

Pressure

Pressure
T

Outlet line temperature

Outlet line temperature
W

Load cells

Load cells
Bioreactors
Minimise selection

The following 46 products correspond to your selection. You haven't found the product? Please contact us.

Separator

Using a centrifuge or separator, the cell culture medium is rotated at high speed and centrifugal force is used to separate the mixture into its denser and lighter components. With the aid of pressure sensors, the inlet and outlet can be monitored and thus the separation performance optimized.

For the process equipment used during purification, such as separators, chromatographs and filters, WIKA has a range of in-line instrumentation that can be integrated optimally into the pipework in accordance with hygienic design requirements.


Separator × P

Supply line pressure

Supply line pressure
T

Supply line temperature

Supply line temperature
P

Outlet line pressure

Outlet line pressure
T

Outlet line temperature

Outlet line temperature
Separator
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The following 11 products correspond to your selection. You haven't found the product? Please contact us.

Filtration

Filtration is an important part of the manufacturing process with the aim of product purity. A number of different filtration processes take place during the downstream process. An important quality aspect here is filter monitoring using differential pressure. This makes it possible to detect a filter perforation in good time or to determine when the filter cartridge needs to be changed.

For the process equipment used during purification, such as separators, chromatographs and filters, WIKA has a range of in-line instrumentation that can be integrated optimally into the piping in accordance with hygiene requirements.


Filtration × P

Supply line pressure

Supply line pressure
T

Storage vessel temperature

Storage vessel temperature
P

Filter monitoring

Filter monitoring
P

Storage vessel pressure blanket

Storage vessel pressure blanket
P

Pressure indication

Pressure indication
T

Filter temperature

Filter temperature
L

Storage vessel level

Storage vessel level
P

Outlet line pressure

Outlet line pressure
T

Supply line temperature

Supply line temperature
P

Supply line pressure

Supply line pressure
P

Filter monitoring

Filter monitoring
T

Storage vessel temperature

Storage vessel temperature
T

Filter temperature

Filter temperature
P

Storage vessel pressure blanket

Storage vessel pressure blanket
Filtration
Minimise selection

The following 30 products correspond to your selection. You haven't found the product? Please contact us.

Chromatography

In bioprocesses, it is common to use liquid chromatography for purification in the downstream process, to separate proteins or biomolecules. The inlet and outlet from the chromatography column can be monitored and adapted optimally to the process with the aid of in-line instrumentation. For the process equipment used during purification, such as separators, chromatographs and filters, WIKA has a range of in-line instrumentation that can be integrated optimally into the piping in accordance with hygiene requirements.


Chromatography × P

Supply line pressure

Supply line pressure
T

Supply line temperature

Supply line temperature
F

Force

Force
P

Outlet line pressure

Outlet line pressure
T

Outlet line temperature

Outlet line temperature
Chromatography
Minimise selection

The following 13 products correspond to your selection. You haven't found the product? Please contact us.

Mobile tanks

Mobile tanks serve various purposes in the downstream process:

  • Internal transport between the different process steps in clean rooms
  • Internal storage
  • Internal transport in buildings
  • Transport between different factories

Particular attention is paid to ensuring that the storage and transport conditions of the process liquids are monitored and complied with. These include, in particular, temperature monitoring and leakage monitoring using the pressure drop method, for which a range of instrumentation is available. Even if the vessel should be completely autoclaved, WIKA has developed special measuring instruments for this purpose that withstand the process conditions of autoclaving. Autoclaving is a sterilisation procedure in which items or equipment are sterilised in saturated steam for at least 20 minutes.


Mobile tanks × L

Level

Level
L

Level

Level
P

Pressure blanket

Pressure blanket
P

Pressure blanket

Pressure blanket
P

Pressure indication

Pressure indication
P

Pressure indication

Pressure indication
T

Temperature sensor

Temperature sensor
W

Load cells

Load cells
Mobile tanks
Minimise selection

The following 27 products correspond to your selection. You haven't found the product? Please contact us.

Formulation

After purification, the manufacturer formulates the therapeutic protein in accordance with the production specifications. These requirements take into account factors such as product stability and dosage form. For this purpose, excipients (pharmacologically inactive ingredients) may be used to improve the medicinal product. These can include colour additives, time release factors and fillers. In addition, substances such as antioxidants, buffers and surfactants can be added to increase the stability of the drug to reduce clumping.

For the inlet and outlet lines, WIKA has a range of in-line instrumentation that can be integrated optimally into the piping in accordance with hygiene requirements.


Formulation × P

Mixing vessel pressure blanket

Mixing vessel pressure blanket
L

Mixing vessel level

Mixing vessel level
P

Mixing vessel pressure blanket

Mixing vessel pressure blanket
L

Mixing vessel level

Mixing vessel level
T

Mixing vessel temperature

Mixing vessel temperature
T

Mixing vessel temperature

Mixing vessel temperature
P

Supply line pressure

Supply line pressure
T

Supply line temperature

Supply line temperature
P

Outlet line pressure

Outlet line pressure
T

Outlet line temperature

Outlet line temperature
W

Load cells

Load cells
Formulation
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The following 32 products correspond to your selection. You haven't found the product? Please contact us.

Fill & finish

At the end of production, the product is filled into the appropriate vials, ampoules or syringes. It is then marked until the finished drug is packaged and made ready for shipment in bulk packs.

A range of in-line instrumentation is available for filling lines, which can be integrated optimally in accordance with hygiene requirements. WIKA offers a comprehensive range of measuring instruments for the monitoring of ventilators and blowers, overpressure monitoring for isolator, RABS and clean rooms, temperature monitoring on heat exchangers, measuring of the air flow and the air velocity in air ducts and air-conditioning systems.


Fill & finish × P

Pressure indication of filter monitoring

Pressure indication of filter monitoring
P

Supply line pressure

Supply line pressure
T

Supply line temperature

Supply line temperature
T

Filling line temperature

Filling line temperature
P

Filling line pressure

Filling line pressure
L

Storage vessel level

Storage vessel level
W

Dosing/Weighing

Dosing/Weighing
P

Storage vessel pressure blanket

Storage vessel pressure blanket
P

Pressure Isolator / RABS

Pressure Isolator / RABS
T

Storage vessel temperature

Storage vessel temperature
Fill & finish
Minimise selection

The following 30 products correspond to your selection. You haven't found the product? Please contact us.

CIP

CIP ("Cleaning in place") is an automated cleaning process for process equipment and machinery. All surfaces in the system that come into contact with the product are cleaned without disassembling the system or dismantling essential components.

The key process steps of a CIP cleaning are:

  • Product rinsing to remove residues
  • Prerinsing
  • Alkaline cleaning to remove fats and proteins
  • Intermediate rinsing
  • Acid cleaning to remove mineral residues and to neutralise
  • Second intermediate rinsing
  • Disinfection
  • Finally, rinsing with cold water

The hygienic design of process equipment in contact with products is a prerequisite for CIP and serves to avoid microbiological contamination, and with that, ensuring product quality. WIKA offers a comprehensive programme for the integration of measuring instruments for CIP cleaning as well as instrumentation specifically adapted to the caustic and acid tanks - in corrosion-resistant special materials.


Cleaning in place × P

Pressure

Pressure
P

Supply line pressure

Supply line pressure
P

Pressure indication

Pressure indication
P

Pressure blanket

Pressure blanket
T

Temperature of CIP return

Temperature of CIP return
P

Pressure of CIP return

Pressure of CIP return
P

Pressure of CIP supply

Pressure of CIP supply
T

Temperature of indication

Temperature of indication
T

Temperature of container

Temperature of container
T

Temperature of CIP supply

Temperature of CIP supply
L

Hydrostatic level

Hydrostatic level
W

Load cells

Load cells
Cleaning in place
Minimise selection

The following 40 products correspond to your selection. You haven't found the product? Please contact us.

SIP

SIP ("Sterilise or steam in place") is a time-controlled sterilisation for both the upstream and downstream processes in biopharmaceutical production using pure steam. It is part of a disinfection routine that is carried out after each production batch. It follows the CIP process ("clean in place"). During SIP, care must be taken to ensure that all wetted surfaces in contact with the product are sterilised by the condensing saturated steam. Thus, no air bubbles in any branches or dead spaces should prevent the steam from wetting the surfaces to be sterilised.

WIKA's in-line instrumentation, which can be optimally integrated into the SIP lines in accordance with hygiene requirements, reduces the risks of any dead space and thus contamination. For the highest demands, WIKA has sensors with integrated self-monitoring that immediately signal if the parts in contact with the product are damaged. A wide range of instrumentation is also available for the plant steam in order to monitor the steam precisely.


SIP × P

Steam pressure outlet

Steam pressure outlet
T

Pure steam outlet temperature sensor

Pure steam outlet temperature sensor
T

Indication of the pure steam outlet temperature

Indication of the pure steam outlet temperature
L

Level in the steam generator

Level in the steam generator
P

Plant steam pressure

Plant steam pressure
T

Input temperature of the plant steam

Input temperature of the plant steam
T

Outlet temperature of plant steam

Outlet temperature of plant steam
T

Inlet temperature sensor for ultrapure water

Inlet temperature sensor for ultrapure water
P

Ultrapure water inlet pressure

Ultrapure water inlet pressure
SIP
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The following 41 products correspond to your selection. You haven't found the product? Please contact us.

WFI preparation

Water for injection (WFI) must meet the strictest hygiene standards. It is produced by filtering and distilling drinking water. This purification process leads to a significantly lower germ-load than with tap water. The WFI is circulated constantly via a high-temperature circulation line (65 to 80 °C) to maintain circulation at all points of use of the WFI system. This minimises the possibility of microbial contamination. The specification of the water used as an ingredient for drugs, and for cleaning, must comply with the currently specified quality requirements in accordance with the applicable standards.

WIKA's in-line instrumentation, which can be optimally integrated into the WFI lines in accordance with hygiene requirements, reduces the risks of any dead space and thus contamination. For the highest demands, WIKA has sensors with integrated self-monitoring that immediately signal if the parts in contact with the product are damaged.


WFI preparation × P

Distillation pressure

Distillation pressure
L

Distillation vessel level

Distillation vessel level
T

Distillation temperature

Distillation temperature
P

Supply line pressure

Supply line pressure
T

Supply line temperature

Supply line temperature
T

Outlet line temperature

Outlet line temperature
P

Outlet pressure

Outlet pressure
WFI preparation
Minimise selection

The following 27 products correspond to your selection. You haven't found the product? Please contact us.

Calibration and service centre

Consultation, design, implementation – all from one source

Precise calibration instruments are the starting point for resolving your test requirements. However, they only form one part of a high-performance calibration system. From our extensive product range, we can design a complete and individual solution for you which contains all the relevant components – with adaptability for test items, pressure and vacuum supply, components for pressure control and fine adjustment, through to voltage supply and multimeters for the calibration of electrical test items.

Our particular strength lies in the project planning, development and the building of complete, individual, user-specific systems – from simple manual workstations through to fully automatic test systems in production lines.

Calibration technology and calibration services

Are you looking for suitable calibration equipment for your applications? Get an overview of our wide range of calibrators. In addition, use our calibration service for pressure, temperature, force, flow and also electrical measurement parameters. We calibrate your references and test equipment independently of manufacturers in our accredited calibration laboratories, or directly on your premises.

Additional services

WIKA supports you with additional services – our experts are at your disposal. Service technicians will make sure that your measuring instruments, in case of breakdowns and repairs, are fully functional again in a short space of time. From pressure controllers and diaphragm seal systems to calibration baths – you can get everything from us in the one place. Furthermore, we install your measuring units and provide you with support in the commissioning of the instrumentation. Through our local experts, we can be reached worldwide, are quickly available and tuned to individual circumstances. Try it for yourself.


Calibration and service centre × CP

Pressure calibration

Pressure calibration
CT

Temperature calibration

Temperature calibration
Calibration and service centre
Minimise selection

The following 25 products correspond to your selection. You haven't found the product? Please contact us.